Effects and Mechanisms of Ursolic Acid on Inducing Apoptosis of Human Esophageal Carcinoma Cell Line Eca-109
- VernacularTitle:熊果酸诱导人食管癌细胞Eca-109凋亡的作用及机制
- Author:
Guoqing CHEN
;
Yi SHEN
;
Hong DUAN
;
Weixue TANG
;
Yulong CHEN
- Publication Type:Journal Article
- Keywords:
Ursolic acid;
Human esophageal carcinoma cell line Eca-109;
Apoptosis;
Flow cytometry;
P27kip1
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects and possible mechanisms of ursolic acid (UA) on inhibiting proliferation of human esophageal carcinoma cell Eca-109 and inducing its apoptosis. Methods Cell proliferation was determined by MTT assay. Electron microscope was used to observe the ultrastructural changes of Eca-l09 induced by UA. Cell cycle and apoptotic rate were analyzed by flow cytometry (FCM),and the expression of P27kip1,Bcl-2 and Bax were detected by Western blot method. Results UA could significantly inhibit the growth of Eca-109 cells(P
