Determination of the Content of Caffeic Acid in Chanhou Tieshijin by HPLC
- VernacularTitle:高效液相色谱法测定产后贴湿巾中咖啡酸的含量
- Author:
Junxia CHEN
;
Ling BIAN
- Publication Type:Journal Article
- Keywords:
Chanhou Tieshijin;
HPLC;
caffeic acid;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish HPLC method for the determination of the content of caffeic acid in Chanhou Tieshijin. Methods The determination was performed on HangBang C18 column (200 mm?4.6 mm, 5 ?m). The mobile phase was methanol-buffer phosphate (23∶77) at a flow rate of 1.0 mL/min. The UV detection wavelength was set at 323 nm and the sample size was 10 ?L. Results The linear range of caffeic acid was 0.052～0.26 ?g (r=0.999 8), with average recovery rate of 97.44% (RSD=0.78%). Conclusion The established method is simple, rapid and accurate, and suitable for the quality control of Chanhou Tieshijin.
