Effect of Zhibo Dihuang Pill on Leptin-induced Idiopathic Central Precocious Puberty in Mice
- VernacularTitle:知柏地黄丸对瘦素诱导特发性性早熟模型小鼠的影响
- Author:
Mengyuan LIU
;
Wen XU
;
Liuying XIAO
;
Chao HAN
- Publication Type:Journal Article
- Keywords:
ZHIBO DIHUANG PILL/pharmacology;
PRECOCIOUS PUBERTY/TCD therapy;
LUTEINIZING HORMONE/blood;
ESTROGEN/blood;
DISEASE MODELS,ANIMAL;
MICE
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2004;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Zhibo Dihuang Pill(ZDP) on leptin-induced idiopathic central precocious puberty(ICPP) in mice.Methods Eighteen BALB/c female mice aged 18 months were randomized into 6 groups: normal group,model group,high-and low-dose ZDP groups(at the dose of 101.4 and 50.7 g?kg-1?d-1 respectively),and megestrol acetate(3.9 mg?kg-1?d-1)group and gonadotropin releasing hormone analogues(GnRH-A) group(at the first dose of 1573.5 ?g/kg,the second and the third dose of 1180 ?g/kg,once every other day).Except the normal group,the mice in other groups received intraperitoneal injection of leptin 20mg/kg to establish ICPP model.After treatment,the vaginal opening,body weight,uterus weight and ovaries weight as well as the serum levels of luteinizing hormone(LH),estrogen(E2) were detected.Meanwhile,the uterus index and ovaries index were also examined.Results High-dose ZDP had an inhibition on the advance of vaginal opening induced by leptin after modeling for 4~6 days(P0.05).Conclusion The therapeutic effect of ZDP for ICPP is probably related with the inhibition of the advance of hypothalamic-pituitary-gonad axis activation.
