Content Determination of Hesperidin in Shuhouyin by HPLC
- VernacularTitle:高效液相色谱法测定术后饮中橙皮苷的含量
- Author:
Gang WANG
;
Liangcun ZHAO
- Publication Type:Journal Article
- Keywords:
HPLC;
Shuhouyin;
hesperidin;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for the content determination of hesperidin in Shuhouyin. Methods Chromatographic assay was performed on CenturySIL C18-AQ (250 mm?4.6 mm, 5 ?m) column at room temperature. The mobile phase was acetonitrile-0.2% phosphoric acid solution (21∶79). The flow rate was 0.8 mL/min. The detection wavelength was at 284 nm. The sample size was 20 ?L. Results Good linear relationship of hesperidin achieved in the range of 1.004～10.04 ?g. The average recovery rate was 98.59% with RSD=0.72%. Conclusion The method is simple, accurate and stable with good reproducibility. This method can be used for determination of hesperidin in Shuhouyin.
