Total flavonoid extracted from Sophara japonica buds with assistant of laccase from endophytic Fusarium sp. C-8
- VernacularTitle:内生镰刀菌漆酶辅助提取槐米中总黄酮的研究
- Author:
Yunfeng XU
;
Zhen ZHANG
;
Jianqin ZHOU
;
Jinglei CHEN
;
Jianwen WANG
- Publication Type:Journal Article
- Keywords:
the buds of Sophara japonica L.;
endophytic Fusarium sp. C-8;
laccase;
total flavonoids;
enzyme-assistant extraction
- From:
Chinese Traditional and Herbal Drugs
1994;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the application of extracellular laccase from endophytic fungi to total flavonoid extraction. Methods The extracellular laccase vitality of an endophytic Fusarium sp.C-8 from Artemisa annua reached 36.1 U/mL when the fungus was cultivated in revised PDA medium with initial pH 7.0 at 20℃ on a rotary shaker incubator for 6 d. The crude laccase from the cultural medium was applied for extraction of flavonoid from the buds of Sophara japonica. The optimization on the ratio of dry buds to crude enzyme liquid,temperature,time,and pH value was carried out during the process of laccase-assistant treatment. Results After incubation with 40∶1 of crude enzyme (pH 7.0) at 20℃ to dry buds for 1 h,the extraction rate of total flavonoids was 11.4 %,a more increase of 28.7 % than that in regular extraction process. Conclusion The results present a practical method of laccase-assistant extraction process on total flavones in S. japonica buds.
