Effect of APS on recoverable ability from cryopreservation damage of UCB hematopoietic cells
- VernacularTitle:当归多糖对脐血造血细胞冷冻损伤的可恢复性研究
- Author:
Hui YANG
;
Hong WU
- Publication Type:Journal Article
- Keywords:
angelica polysaccharides (APS);
umbilical cord blood (UCB);
hematopoietic cells;
cryopreservation
- From:
Chinese Traditional and Herbal Drugs
1994;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of angelica polysaccharides (APS) on recoverable ability from cryopreservation damage of umbilical cord blood (UCB) hematopoietic cells and APS with hematopoietic growth factors (HGFs) on in vitro expansion of UCB mononuclear cells (MNC) after thawing. Methods Thawed UCB MNC were cultured 24 h or 14 d with various concentration of APS (0,50,100,200,and 400 ?g/mL) or with APS and HGFs. The MNC counting assay,typan blue exclusion assay,colorimetric MTT assay for cell proliferation,CFU-Mix colong-forming assay,and flow cytometry for CD34+ cell rate were detected. Results After adding certain concentration APS in thawed UCB cells,the number of MNC,the rate of trypan blue exclusion,the proliferation of MNC,and the colony production of CFU-Mix were significantly enhanced (P
