Callus induction and cultivation of Phyllanthus urinaria
- VernacularTitle:叶下珠愈伤组织的诱导与培养
- Author:
Li CHANG
;
Jianping XUE
- Publication Type:Journal Article
- Keywords:
Phyllanthus urinaria L.;
tissue-culture;
callus
- From:
Chinese Traditional and Herbal Drugs
1994;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish culture method for Phyllanthus urinaria. Methods To study the possible effective factors of culture condition by comparing with different explants,sucrose,plant growth substance,and its ratio. Results The inductivity of stem was the highest about 55.56%,but callus of leaves could not be induced.6-BA was the most important factor on callus induction,followed by 2,4-D NAA and sucrose. Conclusion The optimal medium to induce and subculture is MS+2,4-D 0.5 mg/L+6-BA 1.0 mg/L+NAA 0.1 mg/L+sucrose 10 g/L.
