Determination of Berberine Hydrochloride in Coptis chinensis Franch.and Xianglian Pills by Thin Layer Chromatograph-Fluorescence Spectrophotometry Method
- VernacularTitle:薄层色谱-荧光分光光度法测定黄连及香连丸中盐酸小檗碱的含量
- Author:
Xinxin WEN
;
Danhua LIU
;
Chenhuan YU
- Publication Type:Journal Article
- Keywords:
Coptis chinensis Franch.;
Xianglian Pills;
fluorescence spectrophotometry;
berberine hydrochloride;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a determining method for the content of berberine hydrochloride in Coptis chinensis Franch.and Xianglian pills.Method The sample was extracted and developed,the berberine hydrochloride was determined by thin layer chromatograph-fluorescence of spectrophotometry with Benzen-ethylaceate-isopropanol-methanol-ammonia water(6:3:1.5:1.5:0.5) as the developing system and Ex=365 nm,Em=409 nm.Result The calibration curve was linear in the range of 0.473～ 3.784 ?g,r=0.999 3.The recovery rate in Coptis chinensis Franch.was 99.03%,RSD=1.86%,and in Xianglian pills was 97.10%,RSD=1.09%.Conclusion The method is simple and accurate.
