RAPD and SCAR Markers Analysis on the Indeterminate Growth Mutant of Tomato Derived by Space Mutagenesis
- VernacularTitle:航天诱变番茄无限生长突变体RAPD及SCAR分子标记研究
- Author:
Yanfang WANG
;
Shiheng WANG
;
Yanhong ZHAO
;
Jirong ZHENG
;
Shuijin ZHU
- Publication Type:Journal Article
- Keywords:
space mutagenesis;
tomato;
indeterminate growth mutant;
randomly amplified polymorphic DNA;
sequence characterized amplified region
- From:Space Medicine & Medical Engineering
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the indeterminate growth mutant of tomato derived by space mutagenesis to provide the basis for selecting and cultivating the molecular markers of tomato growth habit. Methods Fifty 10-mer randomly amplified polymorphic DNA(RAPD) primers were used to examine the polymorphism of M1 and 10-3-2. Their polymorphic fragments were cloned, and then were transferred to SCAR markers. Results Of all the 50 10-mer RAPD primers, 44 primers amplified polymerase chain reaction(PCR) products and 2 primers (S165 and S168) amplified stable reproducible polymorphic products. The molecular weight of the specific amplified products were 300 bp and 1 500 bp respectively, therefore they were named as TRS165300 and TRS1681500 temporarily. And TRS1681500 was transferred into stable sequence characterized amplified region(SCAR) marker and this marker could be a specific genetic marker of this indeterminate growth habit mutant. Conclusion Space mutation can produce mutants at DNA level from the loaded materials. The indeterminate growth mutant of tomato is derived by space mutagenesis which can provide a valuable material for studying the regulation and control of tomato growth habit.
