HPLC Fingerprint of Radix Gentianae
- VernacularTitle:龙胆药材的HPLC指纹图谱研究
- Author:
Weixin JIANG
;
Hao QIN
;
Wenshun HE
- Publication Type:Journal Article
- Keywords:
Radix Gentianae;
HPLC;
fingerprint;
quality control
- From:
Chinese Traditional and Herbal Drugs
1994;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the HPLC fingerprint of methanol-resoluble component in Radix Gentianae collected from different habitats and accordingly offer the evidence for quality control and quality evaluation of Radix Gentianae.Methods Ten batches of Radix Gentianae were measured by the RP-HPLC,and their fingerprints were obtained.Chromatographic condition: ODS-column,gradient elution with methanol-water,the detection wavelength was at 254 nm,flow rate was at 1.0 mL/min,and the column temperature was 25 ℃.Results The mutual mode of Radix Gentianae was set up and then the similarity was compared with software which was published by the Committee of Pharmacopeia of China.Conclusion According to the analysis of statistics,this method is steady,accurate with good repeatability,and can be used as standardization implantation and quality control of Radix Gentianae.
