THE RELATIONSHIP BETWEEN THE INSTABILITY OF PINX1 GENE AND GASTRIC CARCINOMA
- VernacularTitle:内源性端粒酶抑制基因遗传不稳定性与胃癌进展的关系
- Author:
Liang WU
;
Dezhong ZHANG
;
Zerong SHU
;
Guorong ZHENG
- Publication Type:Journal Article
- Keywords:
Gastric carcinoma;
PINX1 gene;
Loss of heterozygosity;
Immunohistochemistry;
Computer imaging techniques;
Human
- From:
Acta Anatomica Sinica
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine loss of heterozygosity(LOH) and microsatellite instability(MSI) of locus D8S277 on chromosome 8 and their influence on the expression of PINX1 in the gastric carcinoma,which may provide an experimental basis for the mechanism of PINX1 gene and tumor development.Methods DNA was extracted from formalin-fixed paraffin-embedded tissues.Polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP) and ordinary silver staining were used to study LOH and MSI of locus D8S277.Envision immunohistochemistry and Leica-Qwin computer imaging techniques were used to assess the expression of PINX1.Results The frequency of LOH was significantly higher in the cases with lymph node metastasis than in those without metastasis(21.15% vs 0,P
