Effect of 1,25-dihydroxyvitamin D_3 on renal expression of TGF-1 and HGF in type 1 diabetic rats
- VernacularTitle:活性维生素D_3对糖尿病大鼠肾脏TGF-?_1和HGF表达的影响
- Author:
Lei LIU
- Publication Type:Journal Article
- Keywords:
Streptozotocin diabetes;
1,25-dihydroxyvitamin D3;
TGF-?1;
HGF
- From:
Journal of Chongqing Medical University
2007;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of 1,25-dihydroxyvitamin D3 on renal expression of TGF-?1 and HGF in type 1 diabetic rats.Methods:30 Sprague-Dawley rats were randomized into 3 groups:normal control rats(group NC),diabetic rats(group DM ) and diabetic rats treated with 1,25-dihydroxyvitamin D3(group DC).Kidney-to-body weight ratio(KW/BW),mean glomerular volume(MGV),fractional mesangial area(FMA),urinary albumin excretion rate(UAER)and serum creatinine(Scr)were measured after 12 weeks of treatment,while transforming growth factor-betal(TGF-?1) expression in kidney cortex were observed by immunohistochemistry.The expression levels of TGF-?1 mRNA and HGF mRNA were detected by reverse transcription and polymerase chain reaction(RT-PCR) at the end of the experiment.Results:UAER,Scr,MGV,FMA and KW/BW ratio were significantly higher in the diabetic rats than those in the normal control rats(P
