Effect of two kinds of cardioplegia on cardiomyocyte apoptosis and related protein expressions in rat hearts
- VernacularTitle:两种心脏停搏液对缺血心肌细胞凋亡及凋亡相关蛋白表达的影响
- Author:
Jun QI
- Publication Type:Journal Article
- Keywords:
TTX;
Myocardial ischemia reperfusion injury;
Cardiomytocyte apoptosis;
Bcl-2 protein;
Bax protein
- From:
Journal of Chongqing Medical University
2007;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of two kinds of cardioplegia on cardiomyocyte apoptosis and the related protein expressions in rat hearts. Methods:24 Wistar rats were randomly divided into 3 groups(n=8):Control group,STH-2 group and TTX group. Isolated rat Langendorff and Neely working heart models were estabished. The changes in cardiomyocyte apoptosis were detected by TUNEL.The expression of Bcl-2 and Bax protein were detected by immunohistochemical assay in cardiomyocyte. The expression of Bcl-2 protein in cardiomyocyte was detected by Western blot. Results:Apoptosis index(AI) in STH-2 group(11.20?0.79)% was significantly higher than that in TTX group(6.92?1.10)% and Control group(1.34?0.23)%(P
