Effects of cardioplegia with tetrodotoxin(TTX) on myocardial?-actin and its and mechanism
- VernacularTitle:TTX停搏液对心肌?肌动蛋白的影响及机制研究
- Author:
Lin YAO
- Publication Type:Journal Article
- Keywords:
TTX cardioplegia;
?-Actin;
Calpain;
Myocardial protection
- From:
Journal of Chongqing Medical University
2007;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of cardioplegia with Na+ channel inhibitor tetrodotoxin(TTX)on myocardial?-actin of isolated rat hearts and its and mechanism. Methods:Langendorff and Neely perfusion models were steadily established,then the rat hearts(10 hearts in each group)were arrested by cardioplegia(St. Thomas No. 2 solution,STH-2) in group STH-2,and by cardioplegia(TTX)in group TTX respectively after perfusion for 30 minutes. These arrested hearts were remained for 60 minutes and then underwent reperfusion for 60 minutes.Pre-ischemia and post-ischemia hemodynamic parameters of the rat hearts(HR,CAF,LVESP and recovery rate of pressure change speed)were studied and changes of calpain was detected by immunohistochemistry and myocardial?-actin by Western blot and immunohistochemistry. Results:There was no differences between two groups before myocardial ischemia.But the recovery of myocardial hemodynamic parameters during reperfusoin in group TTX was better than that in group STH-2(P
