Morphology and mechanism research of gastric cancer vasculogenic mimicry
- VernacularTitle:胃癌细胞血管生成拟态的形态学及机制研究
- Author:
Jing ZHANG
- Publication Type:Journal Article
- Keywords:
Gastric tumor;
Vasculogenic mimicry;
Mechanism
- From:
Journal of Chongqing Medical University
2007;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Vasculogenic mimicry(VM) is a new microcirculation form of tumor cells which found resent years. This research observed whether gastric cancer SGC-7901 cells can form vasculogenic mimicry in 3-dimentional culture and had a primer examination on its mechanism. Methods:Put SGC-7901 cells in 3-dimentinal culture and observed if it could form vasculogenic mimicry. Immunohischemistry tested the MMP-2 and MT1-MMP level of 3-dimentional cultured SGC-7901 cells. Observe the effect of PI3K way inhibitor on MMP-2、MT1-MMP level and VM formation of gastric cancer cells. Results:Gastric cancer SGC-7901 cells can form VM in 3-demintional culture. MMP-2 and MT1-MMP highly expressed in gastric cancer cells that took part in the tube structure. PI3K way inhibitor decreased MT1-MMPmRNA and MMP-2 protein level and inhibited VM formation of SGC-7901cells. Conclusion:Gastric cancer SGC-7901 cells had the ability to form vasculogenic mimicry in vitro 3-dimentional culture.One of its mechanism was probably through decreasing MT1-MMP and MMP-2 level.
