The effect of hnRNP K shRNA on K562 cells
- VernacularTitle:hnRNP K shRNA对K562细胞效应的初步研究
- Author:
Lianfen HUANG
- Publication Type:Journal Article
- Keywords:
Heterogeneous nuclear ribonucleoprotein K;
Short hairpin RNA;
Retroviral vector;
Eukaryotic translation initiation factor-4E
- From:
Journal of Chongqing Medical University
2007;0(09):-
- CountryChina
- Language:Chinese
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Abstract:
Objective:To explore the effect of heterogeneous nuclear ribonucleoprotein K(hnRNP K)short hairpin RNA(shRNA)on K562 cells. Methods:hnRNP K shRNA retrovirus was packaged with pSUPER retro hnRNP K shRNA after its identification by enzyme digestion and sequencing analysis,then FCM,Wright staining,MTT assay,RT-PCR were used to analyze its effects on cell cycle and apoptosis,proliferation,hnRNP K and eukaryotic translation initiation factor-4E(eIF4E) mRNA of K562 cells respectively. Results:The sequence of the inserted fragment in pSUPER retro hnRNP K shRNA was correct. Compared with the control K562 cells,hnRNP K shRNA treated group showed significantly more cells at G2/M phase(P
