Inhibition of VEGF antisense oligonucleotides on human bladder carcinoma in nude mice
- VernacularTitle:VEGF反义寡核苷酸对裸鼠膀胱癌移植瘤的抑制作用研究
- Author:
Yu GAO
- Publication Type:Journal Article
- Keywords:
VEGF;
Antisense oligonucleotides;
Bladder carcinoma
- From:
Journal of Chongqing Medical University
2007;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the inhibiting effect of VEGF antisense oligonucleotides carried by liposome on the growth of human bladder carcinoma in nude mice. Methods:The nude mice model of human bladder carcinoma was established and VEGF antisense oligonucleotides were carried by liposome to be transfected.The cell proliferation index,microvessel density (MVD) and the expression of VEGF were detected with immunohistochmistry streptavidin-biotin peroxidase(SP) method and the cell apoptosis index was determined with Tunnel technology. Results:The growth inhibitory rate in antisense experimental group and sense experimental group were 71.08% and 7.19% respectively. The differences in the cell proliferation index,apoptosis index,microvessel density(MVD) and the expression of VEGF between the antisense experimental group and the sense experimental group or the control group had significances. There was no statistical significance in the detection indexes between the sense experimental group and the control group. Conclusions:The VEGF antisense oligonucleotides can inhibit the neovascularization and the growth of bladder carcinoma in nude mice,which might be an important gene therapeutic strategy for the bladder carcinoma in future.
