Fingerprint of Desmodium styracifolium by HPLC
- VernacularTitle:广金钱草HPLC指纹图谱的研究
- Author:
Sini CHEN
;
Zhenqiu ZHANG
- Publication Type:Journal Article
- Keywords:
Desmodium styracifolium;
fingerprint;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: The fingerprint chromatograms were established for quality evaluation of Desmodium styracifolium collected from 11 areas by HPLC. METHODS: The analysis was performed on a Phenomsil C_(18) column((4.6 mm)?250 mm,5 ?m) with acetonitrile-carbinol-water as mobile phase in a gradient mode at a flow rate of(1.0) mL/min,and at a column temperature of 25℃.The detection of wavelength was at 272 nm. RESULTS: 11 peaks were selected as the common fingerprint peaks.The relative standard deviations for relative retention values and relative peak areas were less than 3% in the precision and repeated test,the similarity of 11 batches of samples were no less than 0.9. CONCLUSION: The method is reliable and can be helpful on the quality control of Desmodium styracifolium.
