RP-HPLC fingerprint of herba Taraxaci in Henan Province
- VernacularTitle:河南蒲公英RP-HPLC指纹图谱研究
- Author:
Xifeng LI
;
Huimin SHI
;
Yan WAN
- Publication Type:Journal Article
- Keywords:
herba Taraxaci;
RP-HPLC;
fingerprint chromatogram
- From:
Chinese Traditional Patent Medicine
1992;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish a normal HPLC fingerprint chromatogram of herba Taraxaci in Henan Province. METHODS: 40 different kinds of herba Taraxaci were determined by RP-HPLC.Methanolthe solution of NaH_2PO_4(0.02 mol/L)(pH=3.8) gradient elution were adopted as a mobile phase,80 min the recording chromatogram chart and 1.0 mL/min of the velocity of flow detection wavelength was at 323 nm,column temperature was at 35℃. RESULTS: 9 steady mutual peaks were indicated. CONCLUSION: This method is simple,credible and of good reproducibility,can be used to identify and evaluate herba Taraxaci.