Effects of Radix inulae in Guanxi Suhe Pill on cardiomyocytes injury induced by H_2O_2
- VernacularTitle:冠心苏合丸原方及改进方对心肌细胞过氧化氢损伤的影响
- Author:
Yikui LI
;
Jinyan ZHANG
;
Lianda LI
- Publication Type:Journal Article
- Keywords:
Guanxi Suhe Pill;
cardiomyocyte;
hydrogen peroxide-induced injury;
aristolochic acid;
Aristolochia debilis sieb.et Zucc;
Radix inulae
- From:
Chinese Traditional Patent Medicine
1992;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the change of Guanxi Suhe Pill(GXSH) in cardiomyocytes suffering from hydrogen peroxide-induced injury due to replacement of Aristolochia debilis sieb.et Zucc by Radix inulae. METHODS: Myocardial cells from neonatal SD rats were cultured in dulbecco's modified eagle's medium(DMEM).The primary culture cardiomyocytes were injured by hydrogen peroxide(1 mmol/L) for 4 h,and serum containing GXSH was added to the medium at the same time.The creatine kinase(CK) activity,lactate dehydrogenase(LDH) activity and malondialdehyde(MDA) content in culture medium were determined.Intracellular superoxide dismutase(SOD) activity,the activity of Na~+-K~+-ATPase and Ca~(2+)-Mg~(2+)-ATPase in the cardiomyocytes were also observed. RESULTS: GXSH containing Aristolochia debilis sieb.et Zucc,GXSH containing Radix inulae,and GXSH without above both herbs significantly inhibited the release of LDH and CK,decreased the content of MDA and increased SOD activity,increased the volume of Na~+-K~+-ATPase and Ca~(2+)-Mg~(2+)-ATPase of hydrogen peroxide-injured cardiomyocytes.The effects of TG and BG were stronger significantly than those of QG on some parameters. CONCLUSION: GXSH all has a remarkable protective effect on cardiomyocytes suffering from hydrogen peroxide-induced injury without and with Aristolochia debilis sieb.et Zucc or taking the place of the GXSH containing Radix inulae do not influence its protective effects on cardiomyocytes suffereing from hydrogen peroxide-induced injury.
