Assay of ephedrina hydrochloridum and pseudoephedrine hydrochloride in Chuanliting Spray by HPLC
- VernacularTitle:HPLC测定喘立停喷雾剂中盐酸麻黄碱和盐酸伪麻黄碱的含量
- Author:
Shudan ZHANG
;
Zhengyan XIA
;
Ying FENG
- Publication Type:Journal Article
- Keywords:
ephedrina hydrochloridum;
pseudoephedrine hydrochlorid;
Chuanliting Spray;
RP-HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish a RP-HPLC method for determining ephedrina hydrochloridum and pseudoephedrine hydrochloride in Chuanliting Spray(Herba Ephedrae,Folium Artenisiae argyi,Radix et Rhizoma Asari,etc.). METHODS: HPLC conditions were as follows: C_(18)(4.6 mm?250mm,5?m) column,0.2% phosphoric acid—acetonitrile(96∶4) as mobile phase with flow rate of 1mL/min,and the detection wavelength at 205nm.(RESULTS:) The calibration curve of ephedrina hydrochloridum was linear between 0.448-3.416?g(r=(0.999 8,) n=7).The average recovery was 100.26% and RSD=2.17%(n=5).The calibration curve of pseudoephedrine hydrochloride was linear between 0.16-1.12?g(r= 0.999 1,n=7).The average recovery was 101.60% and RSD =2.19%(n=5). CONCLUSION: The method is convenient and efficient,and can be used for quantitative analysis and quality control of this preparation.
