Effect of Kechuanning on Expression of IL-5mRNA in Lung Tissue of Bronchial Asthmatic Rats
- VernacularTitle:咳喘宁对支气管哮喘大鼠肺组织白细胞介素-5mRNA表达的影响
- Author:
Muxiang YANG
;
Wentao YU
;
Huazhou XU
- Publication Type:Journal Article
- Keywords:
Kechuanning;
bronchial asthma;
IL-5;
rat
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe effect of Kechuanning on the expression of IL-5mRNA in lung tissue of bronchial asthmatic rats.Methods Forty rats were randomly divided into 5 groups:the normal group,the model group,Kechuanning high-dose group (27 g/kg),Kechuanning low-dose group (13.5 g/kg),Guilong Kechuanning control group (0.45 g/kg),8 rats in each group.The bronchial asthmatic model was established by egg protein sensibilization and inhalation provocation.The rats of each treatment group were lavage administration each day from the first time of provocation to execution.After 4 weeks of treatment,the rats were killed and lung tissue were taken to dying of HE to be observed.The expression of IL-5mRNA in lung tissue were determined by RT-PCR.Result Compared with the normal group,the thickness of bronchus wall and bronchus smooth muscle,the numbers of eosinophile granulocyte and leukomonocyte,and expression of IL-5mRNA in lung tissue were increased (P
