HPLC Determination of Berberine Chloride in Compound Wuzhigan Capsules
- VernacularTitle:HPLC法测定复方五指柑胶囊中盐酸小檗碱的含量
- Author:
Hong SUN
;
Shuyi HU
;
Jianbo ZHANG
- Publication Type:Journal Article
- Keywords:
compound Wuzhigan capsules;
HPLC;
berberine chloride;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determinate Berberine chloride in compound Wuzhigan capsules and develop a steady and reliable method for the quality control of the capsules.Methods A HPLC method was adopted.The column was Diamonsil C18 (250 mm?4.6 mm,5 ?m) with the column temperature of 30 ℃,the mobile phase was acetonitrile-0.033 mol/L potassium dihydrogen phosphate (30∶70) with the flow rate of 1.0 mL/min,and the detection wavelength was at 347 nm.Results Berberine chloride has a linearity in the range of 2.52～12.6 ?g/mL,and the average content in the capsules was 1.686 mg/capsules.Conclusion This method is sample,accurate,and can be used for the quality control of compound Wuzhigan capsules.
