Determination of paeoniflorin and baicalin in Weichang Heji by HPLC
- VernacularTitle:HPLC测定胃肠合剂中芍药苷和黄芩苷的含量
- Author:
Ming LING
;
Anyu MA
- Publication Type:Journal Article
- Keywords:
HPLC;
paeoniflorin;
baicalin
- From:
Chinese Traditional Patent Medicine
1992;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish a HPLC method for determing paeoniflorin and baicalin in Weichang Heji(Radix Paconiae alba,Radix Scutellariae,Radix et Rhizoma Khei,etc.). METHODS: The column was Ultimate XB-C_(18)(5 ?m,250 mm?4.6 mm),methanol-water-acetic acid(35∶65∶1) as the mobile phase at a flow rate of 1.0 mL/min,the detecting wavelength was at 244 nm. RESULTS: The linear range was within 25 ?g/mL-250 ?g/mL for paeoniflorin and baicalin. The average recovery rate was(98.12%) and 96.81%,respectively.RSD was 1.16% and 0.95%(n=6) respectively. CONCLUSION: The method is simple and accurate.It can be used to determine the contents of paeoniflorin and baicalin in Weichang Heji meantime.
