Effects of PUVA on Apoptosis and Expression of Fas in Leukemia Cells
- VernacularTitle:光化学疗法对人白血病细胞凋亡及Fas表达的影响
- Author:
Nannan CHEN
;
Shilin HUANG
;
Yang XIANG
- Publication Type:Journal Article
- Keywords:
PUVA therapy;
leukemia;
HL-60 cells;
K562 cells;
NB4 cells;
apoptosis;
Fas
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of psoralen (PSO) and long wave ultraviolet A (PUVA) on apoptosis and expression of Fas in HL-60,K562 and NB4 leukemia cells.Methods The cells were incubated with PSO in different concentrations irradiated with or without UVA.The changes of ultrastructure of cells were observed under the electron microscope.The expression of Fas gene was detected by fluorescent quantitation PCR.The apoptosis ratio and the expression of Fas protein were detected through the flow cytometry.The factorial design and analysis of variance were used to analyze the interaction among the factors.Results There were obvious ultrastructure changes about apoptosis in leukemia cells after treated with PUVA.PSO,UVA and PUVA all increased the apoptosis ratio and expression of Fas gene and protein,and the effects of PUVA were stronger than the other two (P
