Expression and role of AQP9 in models of cultured steatosis hepatocytes
- VernacularTitle:水通道蛋白9在培养肝细胞脂肪变性模型中的表达及意义
- Author:
Fengying ZHAO
- Publication Type:Journal Article
- Keywords:
Oleic acid;
Steatosis hepatocyte;
Triglyceride;
FFA;
Glycerol;
AQP9
- From:
Journal of Chongqing Medical University
2007;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the expression and role of AQP9 in the models of cultured steatosis hepatocytes.Methods:Steatosis models of hepatocytes was established by adding oleic acid to the growing L-02 cell strain.The expression of AQP9 mRNA was measured with RT–PCR and the protein expression of AQP9 were measured by Western blotting.Lipid droplets in the hepatocytes were observed by oil red staining and the contents of triglyceride,FFA and glycerol in hepatocytes were measured with analyzed kit.Results:Through oil red staining.a few lipid droplets were observed at 24h and steatosis hepatocyte increased greatly at 72h.Triglyceride,FFA and glycerol contents in hepatocytes of model groups also increased,as compared with the control group(P
