Establishment and analysis of saliva two dimensional gel electrophoresis profiles of Type 2 diabetes patients
- VernacularTitle:2型糖尿病患者唾液双向电泳图谱的建立和分析
- Author:
Linlin SHEN
- Publication Type:Journal Article
- Keywords:
Type 2 diabetes;
Salivary proteomics;
2-DE electrophoresis;
MALDI-TOF-MS
- From:
Journal of Chongqing Medical University
2007;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The purpose of this study was to establish two dimensional gel electrophoresis(2-DE) profiles of saliva of Type 2 diabetes patients,and to identify the differential proteomic expressions between normal persons and Type 2diabetes patients.Methods:Samples of saliva protein were separated by two dimensional electrophoresis with optimization.Differential proteomic expressions between the Type 2diabetes patients and the normal control persons were identified by two dimensional polyacrylamide gel electrophoresis,silver staining,image master 2-DE software analysis,peptide mass fingerprinting based on matrix assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF-MS),Bioworks and NCBI software database searching.Results:The two dimensional polyacrylamide gel electrophoresis profiles of saliva proteins were successfully established by 2-DE.twenty of the significant differential proteins were selected and identified by MALDI-TOF-MS.seven of them were finally identified.Conclusions:The 2-DE profiles of saliva proteins were established and the differential proteomic expressions were identified by proteome technique in our study.This can be an experimental basis for further research of the pathogenesis and treatment of Type 2 diabetes.
