siRNA inhibits HPV16 E6 expression and cell proliferation in HNE-1 cell line
- VernacularTitle:siRNA抑制鼻咽癌HNE-1细胞株HPV16 E6的表达和细胞增殖
- Author:
Na SHEN
- Publication Type:Journal Article
- Keywords:
siRNA;
HPV16 E6;
RNAi;
Nasopharyngeal carcinoma;
Gene silence;
Reverse transcription polymerase chain reaction
- From:
Journal of Chongqing Medical University
2007;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the inhibitory effect of Small interferirng RNA(siRNA) on human papilloma vinus(HPV)16 E6 oncogenes in nasopharyngeal carcinoma(NPC) cell line HNE-1,and observe the effects of HPV16 E6 silencing on NPC cell growth.Methods:Four siRNA against the HPV16 E6 gene were designed and transfected into HNE-1 cell respectively using RNAi-Mate transfection reagent.MTT was used to investigate the cellular proliferation after the transfection.RT-PCR was used to detect the expression of HPV16 E6 and protein level of HPV16 E6 was measured by Immunohistochemical staining.Results:After the most effective siRNA was transfected into HNE-1cell,the result of MTT indicated that the cellular proliferation was restrained remarkably,and the highest inhibitory rate was 32%.Meanwhile,RT-PCR and immunohistochemical staining showed that there was a significant decrease in HPV16 E6 mRNA and protein level.Conclusion:These results demonstrated that siRNA against HPV16 E6 could effectively downregulate HPV16 E6 expression in HNE-1 cell line,and inhibit the cellular proliferation.RNAi has great potential in the treatment of nasopharyngeal carcinoma as a new gene therapy.
