Synergistic induction of apoptosis of HEP-2 cell line by the combination of TRAIL and 5-FU,DDP
- VernacularTitle:TRAIL联合化疗药物诱导喉癌HEP-2细胞株凋亡的研究
- Author:
Xiaoyan YU
- Publication Type:Journal Article
- Keywords:
TRAIL;
Chemotherapy;
HEP-2 cell line
- From:
Journal of Chongqing Medical University
2007;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate whether TRAIL can synergize 5-Fu and DDP to kill HEP-2 cell line.Methods:MTT was used to detecet the cytotoxicity of HEP-2 cell line intervented in 5-FU,DDP,TRAIL,5-FU+TRAIL and DDP+TRAIL.Fluorescence microscope and FCM were used to detecet the apoptosis rate of cell.Results:HEP-2 cell line was insensitive in TRAIL,but the apoptosis rate obviously was upgraded after intervented in 5-FU+TRAIL and DDP+TRAIL.Conclusion:TRAIL in combination with subtoxic dose of 5-FU and DDP can effectively kill HEP-2 cell line.The combination of TRAIL,5-FU and DDP induced apoptosis of HEP-2 cell line.
