Effects of troglitazone on cytokines induced expression of ICAM-1 in human mesangial cell
- VernacularTitle:曲格列酮对炎症诱导系膜细胞细胞间粘附分子1表达的影响
- Author:
Linyan LIU
- Publication Type:Journal Article
- Keywords:
Troglitazone;
Human mesangial cell;
Tumor necrosis factor-?;
Nuclear factor-?B;
Intercellular adhesion molecule-1
- From:
Journal of Chongqing Medical University
2007;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of troglitazone on cell proliferation and expression of intercellular adhesion molecule-1(ICAM-1) induced by tumor necrosis factor-?(TNF-?) in human mesangial cells.Methods:Human mesangial cells were cultured in RPMI-1640 media containing TNF-? with or without troglitazone.Cell proliferation was assessed by MTT;the mRNA and protein expression of ICAM-1 were measured by RT-PCR and ELISA;the protein expression of NF-?B was measured by immunohistochemistry.Results:Troglitazone inhibited the proliferation of mesangial cell.The mRNA and protein expression of ICAM-1 of the cell induced by TNF-? increased significantly(P
