HPLC fingerprint analysis of the different parts of Herba pogostemonis
- VernacularTitle:广藿香不同部位HPLC指纹图谱的比较研究
- Author:
Yuechun HUANG
;
Gang WEI
;
Xue YIN
- Publication Type:Journal Article
- Keywords:
Herba pogostemonis;
different parts;
fingerprint;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To establish the method of fingerprint-analyzing Herba pogostemonis by HPLC,and compare the variability of the different parts,including stems and leaves,which could be used for quality evaluation of Herba pogostemonis. METHODS: HPLC with Hypersil ODS column was used,the acetonitrile-0.05% phosphoric acid(gradient elution)as a mobile phase and detection wavelength was at 320 nm,column temperature was at 30 ℃,and flow rate was 1.0 mL/min. RESULTS: 16 common peaks were separated on HPLC fingerprint in the stems and leaves of Herba pogostemonis,there was significant variability between the stems and leaves,the content in the leaves was more than in the stems. CONCLUSION: The method is reliable,accurate and provides a reference for the quality control of Herba pogostemonis.
