Determination of three compounds in Qingre Mingmu Tea by RP-HPLC
- VernacularTitle:RP-HPLC同时测定清热明目茶中3个成分的含量
- Author:
Wenting ZHANG
- Publication Type:Journal Article
- Keywords:
Qingre Mingmu Tea;
emodin;
chrysophanol;
physcion;
RP-HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To develop a RP-HPLC method for simultaneously determining three major compounds in Qingre Mingmu Tea(Semen Cassiae,Flos crysanthemi,etc),namely emodin,chrysophanol and physcion with UV detection wavelength at 254 nm. METHODS: The column was Diamonsil C_(18)(250 mm?4.6 mm i.d,5 ?m).The separation was carried out with a gradient program.The mobile phase was acetonitrile-0.1% phosphoric acid at a flow rate of 1.0 mL/min. RESULTS: The standard curve was rectilinear in the range of 5.335213.4 ng(r=(0.999 8)) for emodin,9.075-363.0 ng(r=0.999 8) for chrysophanol,and 4.688-187.5 ng(r=1.000 0) for physcion,respectively.The recoveries of the markers listed above were 96.0%,106.5% and 98.1%,respectively. CONCLUSION: The method is simple,rapid and suitable for the determination of three compounds in Qingre(Mingmu) Tea.
