Comparative Proteomics Analysis on Splenic Lymphocytes in Migraine Rat Model with Hyperactivity of Liver-yang
- VernacularTitle:偏头痛肝阳上亢型大鼠脾脏淋巴细胞的比较蛋白质组学分析
- Author:
Jianjun HU
;
Zeqi CHEN
;
Guangwei ZHONG
- Publication Type:Journal Article
- Keywords:
proteomics;
migraine;
hyperactivity of liver-yang;
splenic lymphocytes;
rat
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the molecule mechanisms of migraine with hyperactivity of liver-yang related proteins. Methods Animal model of migraine with hyperactivity of liver-yang was established through electrical trigeminal ganglion stimulation,and by administering orally with Fuzi decoction. The total proteins of splenic lymphocytes in the rats were separated by immobilized pH gradient-based two-dimensional gel electrophoresis. With coomassie blue staining,the 2-DE images were analyzed by PDQuest 7.0 software. Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF-MS) and database were used to identify the differential proteins. Result Ten differential spots protein were identified,and they were chloride intracellular channel 1,thioredoxin peroxidase Ⅰ,thioredoxin peroxidase Ⅱ,sequence 7 from patent WO9709348,Rho GDP dissociation inhibitor (GDI) alpha,actin related protein 2/3 complex neurofilament light polypeptide,proteasome subunit beta type and heat shock protein-27,annexin A1. Conclusion These protein may be involved in the development of migraine with hyperactivity of liver-yang. These data provide useful clues for elucidating the mechanisms of migraine with hyperactivity of liver-yang.
