Determination of Stachydrine Hydrochloride in Different Parts of Herba Leonuri by HPLC
- VernacularTitle:RP-HPLC法测定益母草不同药用部位中盐酸水苏碱的含量
- Author:
Zhe TANG
;
Li LIU
;
Wei ZHANG
- Publication Type:Journal Article
- Keywords:
Herba Leonuri;
stachydrine hydrochloride;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To control the quality of Herba Leonuri by studying the content of stachydrine hydrochloride in different parts of it. Method A HPLC method was developed for the content detetmination of stachydrine hydrochloride in flower, stem, leafage from Herba Leonuri. Spherisorb SCX colum was used with mobile phase of 20 mmol/L sodium dihydrogen phosphate (containing 0.04% triethylamine and 0.15% phosphoric acid). The colum temperature was 25 ℃, the detective wavelength was 192 nm. Results Stachydrine hydrochloride content was the highest in leafage and the lowest in stem. Conclusion To ensure the quality of Herba Leonuri, it is significant to choose medical material with more leafage.
