EXPRESSION OF Polo-LIKE KINASE 1 DURING THE DIFFERENTIATION OF CARDIOMYOCYTES IN RATS
- VernacularTitle:Polo样激酶1在大鼠心肌细胞分化过程中的表达
- Author:
Jing DI
;
Shuling BAI
;
Lingzhi ZHANG
;
Jie GAO
;
Hao TONG
- Publication Type:Journal Article
- Keywords:
Polo-like kinase 1;
Cardiomyocyte;
Cell cycle regulation;
RT-PCR;
Western blotting;
Immunofluorescence;
Rat
- From:
Acta Anatomica Sinica
1957;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the mechanism of how cardiomyocytes exit from the cell cycle,we examined the expression of polo-like kinase 1(plk1) in the postnatal developmental process of cardiac myocytes. Methods Mitotic Index(MI) of cardiomyocytes was examined in the neonatal,2-week-old,4-week-old,and adult rat hearts(five cases per groups) by double immunofluorescence stained with H3P and ?-sarcomeric actin antibodies.plk1 mRNA and protein expression during the postnatal developmental process of cardiac myocytes were detected by RT-PCR and Western blot analysis in rat hearts. Results The MI of cardiomyocytes in 0-day-old hearts(0.905?0.087%) was approximately 2.4 times over that in 2-week-old hearts(0.372?0.094%)(P
