Cloning and expression of escherichia coli colonization factor antigen CFA/Ⅰ in escherichia coli DH5?
- VernacularTitle:产肠毒素大肠杆菌定居因子Ⅰ基因克隆与表达
- Author:
Xueping HUANG
- Publication Type:Journal Article
- Keywords:
ETEC;
pBV220;
CFA/Ⅰ
- From:
Journal of Chongqing Medical University
2007;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To clone and express colonization factor antigen Ⅰ(CFA/Ⅰ) of enterotoxigenic Escherichia coli (ETEC) in E.coli DH5?. Methods:CFA/Ⅰ gene was cloned by PCR from H10407 and recombined to a expression vector pBV220.This vector was designated pBV220- CFA/Ⅰand transformed into E.coli DH5?. The product was tested by SDS-PAGE and immunogenesis test. Results:The CFA/Ⅰ gene could be expressed in E.coli and contained strong immunogenesis by immune test. Conclusion:This study paved the way for using ETEC CFA/Ⅰ recombinant as a vaccine candidate in future.
