RP-HPLC determination of triptolide in triptolide gel
- VernacularTitle:RP-HPLC法测定雷公藤甲素外用凝胶中雷公藤甲素的含量
- Author:
Yan LI
- Publication Type:Journal Article
- Keywords:
HPLC;
Triptolide;
Gel
- From:
Journal of Chongqing Medical University
2007;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop an HPLC method for the assay of the content of triptolide in triptolide gel. Methods:The content of triptolide was measured by HPLC on Diamonsil C18 column(250mm?4.6mm i.d,5?m)using methanol-water(46∶54,v/v)as mobile phase,at a flow rate of 1.0ml/min. The UV detection was set at 220nm. Results:The calibration curve was proved to be linear in the range of 10.24~51.20?g/ml with a regression coeficient of 0.999 7. The average recovery of the method was 98.3%;RSD was 0.23%(n=5). Conclusion:This method is found to be rapid and accurate for the measurement of triptolide in triptolide gel.
