Preparation and biological characteristics of renal cell carcinoma-dendritic cell fusion vaccine
- VernacularTitle:肾癌-树突状细胞融合瘤苗的制备及生物学特性
- Author:
Zhi HU
- Publication Type:Journal Article
- Keywords:
Dendritic cell;
Cancer vaccine;
Cell fusion;
Renal cell carcinoma
- From:
Journal of Chongqing Medical University
2007;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare renal cell carcinoma-dendritic cell fusion vaccines,and observe the biological characteristics of the fusion cells,to provide theoretical basis and experimental data for further research of DC based cancer vaccines. Methods:The renal cell carcinoma and dendritic cells were fused by using polyethylene glycol(PEG). The growth curve of fusion cells,morphological characteristics,expression of costimulation molecules,abilities to amplify T lymphocytes and grow into new tumors were detected. Results:Renal cell carcinoma and dendritic cells could be fused effectively by PEG.The fusion cells had weak proliferation abilities and were unable to grow into new tumors in immunol-deficiency animals,but the costimulation molecules expression such as CD86 and HLA-DR and the abilities to irritate the proliferation of T lymphocytes were remarkably increased than that of parental control groups(P
