Relationship of the expression of IL-2 and IL-4 in breast cancer with chemosensitivity
- VernacularTitle:乳腺癌中IL-2、IL-4表达与化疗药物敏感性关系的研究
- Author:
Bing YU
- Publication Type:Journal Article
- Keywords:
Breast cancer;
IL-2;
IL-4;
Chemosensitivity
- From:
Journal of Chongqing Medical University
2007;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the relationship of the expression of IL-2 and IL-4 in breast cancer cells with chemosensitivity. Methods:Surgical samples of breast cancer,diagnosed by pathology,were obtained from The First Affiliated Hospital ofChongqing Medical University. The cancer sample cells were cultured separately in the incubator at 37℃,5%CO2 in vitro. The rate of inhibition of cancer cells by 4 kindsof anticancer drugs,which were EADM,5-Fu,NVB and DDP,were assayed by MTT method. Immunohistochemistry was used to detect the expression of IL-2 and IL-4 in the breast cancer tissues. Results:Sensitivity rates of 20 breast cancer cells in 0.1?PPC within 48h in vitro were 30%(EADM),20%(5-Fu),45%(NVB)and 25%(DDP).Respectively the rate of inhibition of EADM,5-Fu,NVB and DDP were significantly higher in the IL-2 positive cancer cells than in the IL-2 negative cancer cells. A positive correlation was found between expression of IL-2 and chemosensitivity for all the 4 anticancer drugs. The rates of inhibition of EADM and NVB were significantly lower in the IL-4 positive cancer cells than in the IL-4 negative cancer cells. A negative correlation was found between expression of IL-4 and chemosensitivity for the 2 anticancer drugs. Conclusions:The expression of IL-2 and IL-4 are related with the chemosensitivity of the breast cancer cells,and they may become one of the markers for judging the effect of chemotherapy in clinic. The combined detection of IL-2 and IL-4 may be taken as an index of the selection of anticancer drugs.
