Determination of Piperine in Mongolian Recipe of Haodaodun-10 by HPLC
- VernacularTitle:HPLC法测定蒙药浩道墩-10丸中胡椒碱的含量
- Author:
Shuyan WANG
;
Haishan XI
- Publication Type:Journal Article
- Keywords:
Haodaodun-10;
Piperine;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a RP-HPLC method for quantitative determinat ion of the piperine in the Mongolian recipe of Haodaodun-10. Methods The determ ination was performed on Shim-pack VP-ODS(150 mm?4.6 mm,5 ?m)column and the mobile phase was a mixture of water and methanol (23 ∶77) with a flow rate of 0.8 mL/min at room temperature. The UV detection wavelength was 343 nm. Results The linear range of piperine was 0.5～5.0 ?g,r=0.999 8.The mean recovery was 9 8.1 %( RSD=1.76 %,n=5). Conclusion The method is simple,accurate and sensiti ve,and can be used for the quality control of Haodaodun-10.
