Improvement of Guangdong Liangcha Granules on Restraint-stress-induced Perox idation in Genital Organs of Mice
- VernacularTitle:广东凉茶颗粒对应激小鼠生殖器官过氧化状态的改善作用
- Author:
Li BAO
;
Xinsheng YAO
;
Rongrong HE
;
Yuanbo LI
- Publication Type:Journal Article
- Keywords:
Guangdong Liangcha Granules;
Restraint Stress;
Testis;
Ovaries;
Glut athione;
Oxygen Radical Absorbance Capacity (ORAC)
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the improvement of Guangdong Liangcha Granules (GLG) on restraint-stress-induced peroxidation in genital organs of mice. Meth ods Mice models of peroxidation injury in genital organs were induced by 18-hou r restraint stress. Testicular and ovarian malondialdehyde (MDA) level was detec ted by thiobarbituric acid method,glutathione (GSH) content by HPLC,xanthine o xidase (XOD) and GSH-PX activities by colorimetric method,nitric oxide (NO) co ntent by Griess chemical method and oxygen radical absorbance capacity (ORAC) by enzyme-linked fluorescent immunoassay. Results Compared with model group,GLG can markedly reduce MDA level,XOD acitivity and NO contents,in addition,GLG c an effectively increase the ORAC value,GSH content,GSH-PX activity in testis and ovaries. Conclusion Oral treatment of Guangdong Liangcha Granules was found to reduce the status of peroxidation in testis and ovaries,and the improvement may be related to the increase of its free radical scavenging activity and lipid peroxidation.
