Study on the Determination of Hypaconitine and Mesaconitine in Shubining Granu les by HPLC
- VernacularTitle:HPLC法测定舒痹宁颗粒中次乌头碱、新乌头碱的含量
- Author:
Zhenwen QIU
;
Dandong LUO
;
Peijian WANG
- Publication Type:Journal Article
- Keywords:
Shubining Granules;
Hypaconitine;
Mesaconitine;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the contents of hypaconitine and mesaconitine in Shubining Granules. Methods The HPLC method was established for the determin ation of hypaconitine and mesaconitine in Shubining Granules. The HPLC was perfo rmed on Akasil C18(4.6 mm?250 mm,5 ?m) column,mobile phase consisted of meth anol-0.1 %triethylamine (70 ∶30),column temperature 30 ℃,and the flow rare was 1.0 mL/min.The detection wavelength was 230 nm.Results A good linearity w as obtained in the range of 0.126～2.520 ?g of Hypaconitine and 0.152～3.04 ?g of Mesaconitine.The average recovery of Hypaconitine was 101.12 %.The average recovery of Mesaconitine was 99.68 %. Conclusion This method is simple and qui ck,and can be used as quality control of Shubining Granules.
