Determination of Ceramide in Mouse Epiderm by Thin-layer Chromatography and G el Image-Forming System
- VernacularTitle:薄层色谱结合凝胶成像系统测定小鼠表皮神经酰胺含量
- Author:
Yan WANG
;
Qing LIN
;
Jinmei XIE
;
Dan HE
;
Qin LI
- Publication Type:Journal Article
- Keywords:
Ceramides;
Content determination;
Thin-layer chromatography;
Gel Im age-Forming System
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for determining the content of ceram ide in skin epidermis. Methods Epidermal lipids of mice were extracted by routin e method,separated by thin-layer chromatography (TLC),and shot by BIO-RAD Ge lDoc system. Epidermal ceramide content was determined by Software of Quantity O ne. Results A good linearity was obtained in the range of 0.416～2.08 ?g (r=0.9 95 3). The precision of the same plate was good,RSD=1.09 %;and the precision of different plates was also good,RSD= 2.10 %. The stability of control soluti on and sample solution was good within 2 h. The reproducibility was good,RSD= 3 .03 %,and the mean recovery rate was 99.58 %and RSD=1.46 %. The RSD of simpl e determination was 3.10 %. Conclusion This method can be used to determine the content of ceramide in skin epidermis.
