Inhibition Effect of Ki67 AS-ODN and Its Combination with Paclitaxel on PC-3 Cells
- VernacularTitle:Ki67反义寡核苷酸及联合紫杉醇对PC-3细胞增殖的影响
- Author:
Xiujing YANG
;
Yan DONG
;
Lian ZHOU
;
Song WU
;
Haibiao LAI
;
Peixun WANG
- Publication Type:Journal Article
- Keywords:
l:Prostate carcinoma cells;
Ki67;
AS-ODN;
Paclitaxel
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibition effect of Ki67 AS-ODN on pr ostate carcinoma PC-3 cells,and its possible synergism existing in combination of Ki67 AS-ODN and paclitaxel.Methods Ki67 AS-ODN were transfected into PC-3 cells by lipofectamine. Cell proliferation was measured by CCK8 method,and Ki6 7 mRNA expression was detected by RT-PCR. The synergetic effect of Ki67 AS-ODN combined with paclitaxel was evaluated by Zhengjun Jin Q method. Results AS-OD N at the concentration of 31.25 nmol /L can significantly inhibit PC-3 cells pr oliferation(P
