HPLC fingerprinting similarity analysis of Radix Paeoniae Alba
- VernacularTitle:白芍HPLC指纹图谱相似度的分析研究
- Author:
Yuefeng LI
;
Xingke YAN
;
Fei SHEN
;
Tingli LI
- Publication Type:Journal Article
- Keywords:
Radix Paeoniae Alba;
paeontflortn;
HPLC;
fingerprinting
- From:
Chinese Traditional Patent Medicine
1992;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish a sensitive and specific HPLC method for controlling the quality of Radix Paeoniae Alba. METHODS: HPLC method was applied for quality assessment of Radix Paeoniae Alba.Column: Hypersil C_(18) column(4.61 mm?200 mm,51 ?m);flow-rate: 1.0 mL/min;column temperature: 25?C.mobile phase: acetonitrile(A) and H_2O(acidified to 0. 05% with phosphoric acid)(B).The concentrations of solvent A were 95%,86%,86% and 65% at 0,25,35,60 min,respectively. RESULTS: The HPLC chromatographic fingerprinting of Radix Paeoniae Alba,showing 14 characteristic peaks,was established from 21 batches of Radix Paeoniae Alba products.The results of similarity analysis was the same as that of herarchical cluster analysis. CONCLUSION: The chromatographic fingerprinting of Radix Paeoniae Alba with high specificity can be used to control its quality and ensure consistency between batches.
