Experimental study on apoptosis of S180 tumor induced by total hedysari polysaccharide
- VernacularTitle:红芪总多糖诱导S180瘤细胞凋亡的实验研究
- Author:
Fengfeng LEI
- Publication Type:Journal Article
- Keywords:
total hedysari polysaccharide;
S180 tumor;
calcium ion;
free radical;
mitochondrial membrane potential
- From:
Chinese Traditional Patent Medicine
1992;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study effects and mechanisms of total hedysari polysaccharide(THPS)inducing apoptosis of S180 tumor. METHODS: 60 Kunming mice were divided into 5 groups randomly after inoculating S180 tumor.Sample group(S) and cyclophosphamide group(CY) were injected intraperitoneally with normal sodium and cyclophosphamide respectively.Low dose THPS group(H100),moderate dose THPS group(H200),and high dose THPS group(H400) were injected intraperitoneally with low,moderate,and high dose THPS respectively.The mice were killed after being treated for 14 days.Appearance of tumor was observed by electron microscope,and DNA,RNA,intracellular (Ca~(2+)),free radicals,mitochondrial membrane potential of tumor were determined by laser scanning confocal microscope. RESULTS: Moderate dose THPS raised intracellular Ca~(2+),free radicals and reduced mitochondrial membrane potential and DNA,RNA of tumor. CONCLUSION: Moderate dose THPS induces apoptosis of S180 tumor through raising intracellular Ca~(2+),free radicals and reducing mitochondrial membrane potential.
