Analysis of medicine components from Compound Qingkailing in rat serum
- VernacularTitle:大鼠血清中复方清开灵药物原型的HPLC-MS~n和HPLC-TOF-MS分析
- Author:
Liqiong PANG
;
Qingfei LIU
;
Qionglin LIANG
;
Xiaorong RAN
;
Yiming WANG
;
Guoan LUO
- Publication Type:Journal Article
- Keywords:
Qingkailing Injection;
medicine antetype;
HPLC-MS~n;
HPLC-TOF-MS
- From:
Chinese Traditional Patent Medicine
1992;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish methods for identifying the antetype of Compound Qingkailing(Calculus bovis,Concha margaritifera,Radix isatidis,Cornu bubali) in rat serum by HPLC-MS~n and HPLC-TOF-MS. METHODS: Addition of methanol to serum after Qingkailing given intravenously to rats was used to precipitate protein.The HPLC-TOF-MS provided the exact molecular weight and the HPLC-ESI-MS~n provided the m/z of multilevel fragment.The medicine antetypes were identified by combining the two methods. RESULTS: Four main medicine antetypes in rat serum,such as geniposide,baicalein,wogonoside and cholic acid,were identified. CONCLUSION: It is a rapid and exact method that can be used to identify other complicated traditional Chinese medicine in vivo.
