Effect of Anxin Granules on Serum TNF-? and IL-6 Contents and Myocardial Cell Apoptosis in Rats with Heart Failure
- VernacularTitle:安心颗粒对心衰大鼠血清TNF-?、IL-6及心肌细胞凋亡的影响
- Author:
Xianming FANG
;
Zhongyun LANG
;
Bin WEI
- Publication Type:Journal Article
- Keywords:
ANXIN GRANULES/pharmacology;
HEART FAILURE,CHRONIC/TCD therapy;
APOPTOSIS;
CYTOKINES;
MYOCARDIUM/pathology;
DISEASE MODELS,ANIMAL;
RATS
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2000;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the relationship of serum TNF-? and IL-6 contents with myocardial cell apoptosis in the chronic heart failure (CHF)rats,and to explore the therapeutic mechanism of Anxin Granules (AXG)in treating CHF.Methods Fifty female SD rats were randomly divided into five groups:normal control group (A),model group (B),captopril group(C),low-dosage AXG group (D)and high-dosage AXG group (E).CHF models were established by intraperitoneal injection of small-dose of adriamycin,once per week for continuous 6 weeks.Meanwhile,group C was given captopril injection (6.25mgkg-1d-1),and groups D and E received gastric gavage of low-dosage AXG (0.8 gkg-1d-1)and high-dosage AXG (1.6gkg-1d-1)respectively.After 6 weeks of modeling,serum levels of TNF-? and IL-6 of different groups were tested by ELISA,the apoptosis of myocardial cells was tested by TUNEL,and then the apoptosis index(AI)was counted.Results Serum levels of TNF-? and IL-6 were increased in the model group (P
