Effect of Shenfu Injection on Adriamycin-induced Calcium Overload in Myocardial Cells of Rats
- VernacularTitle:参附注射液对阿霉素致心肌细胞钙超载的影响
- Author:
Fuman YAN
;
Lequan ZHOU
;
Yali KANG
;
Xiaoying LI
;
Rongjing LUO
- Publication Type:Journal Article
- Keywords:
SHENFU INJECTION/pharmacology;
ADRIAMYCIN/toxicity;
CALCIUM OVERLOAD;
DISEASE MODELS, ANIMAL;
RATS
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2000;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Shenfu Injection (SI)on adriamycin(ADR)-induced calcium overload in myocardial cells of rats.Methods SD rats were randomized into normal group, saline group, ADR group, ADR+saline group, low-, middle-and high-dosage SI groups (treated with SI 0.3, 0.6 and 1.2gkg-1d-1). Except the normal and saline groups, the rats in other groups were given ADR injection. Meanwhile,SI groups received treatment of SI for two weeks. Three weeks after modeling, the single myocardial cells were isolated. The free Ca2+ concentration was detected by ionic imaging method, and the ionic current through L-type calcium channel in cellular membrane was observed by patch clamp technique.Results The free Ca2+ concentration, the mean open probability of L-type calcium channel in cellular membrane and the mean ionic current through L-type calcium channel were increased in ADR group (P0.05).Conclusion The mechanism of SI counteracting the ADR-induced calcium overload in myocardial cells may be related with preventing external calcium from entering the cells,and with inhibiting the release of internal calcium.
