Effect of Total Flavonoids of Hedysarum Polybotry on Inducing Differentiation of Leukemic Cells
- VernacularTitle:红芪总黄酮对人白血病细胞诱导分化的影响
- Author:
Guangyuan LI
;
Che CHEN
;
Huiyuan CHU
- Publication Type:Journal Article
- Keywords:
Hedysarum polybotry;
flavonoids compound;
HL-60 cell;
C-fos gene
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect and mechanism of total flavonoids of Hedysarum polybotry on induction of differentiation in human leukemia HL-60 cells. Method After the treatment of HL-60 cells with total flavonoids of Hedysarum polybotry, the cell differentiation was detected with NBT reduction method. Cell cycle, CD11b and C-fos were analysed by the flow cytometry. Result The positive rate of NBT reduction and the expression of CD11b were significantly increased. Similar, the expression of C-fos gene was upregulated. The growth of HL-60 cells was arrested at G0/G1 and G2/M phase. Conclusion Total flavonoids of Hedysarum polybotry could induce differentiation of HL-60 cells. Its molecular mechanism might be related to the modulation of gene expressions associated with the proliferation and differentiation, which leads to the inhibition of DNA synthesis.
